Name: Jagan Valluri
Title: Professor
Office: Biological Sciences/COS
Phone: 304-696-2409
Email: valluri at marshall.edu
Name:
Jagan Valluri
Title: Professor
Office: Biological Sciences/COS
Phone: 304-696-2409
Email:
valluri at marshall.edu
Education
Ph.D. Texas A&M University, 1988
M.S. Baylor University, 1984
B.S. Osmania University,1981
Major Areas of Experience
Isolation, purification and characterization of proteins from plants and microorganisms, Enzyme kinetics, Carbohydrate analysis by HPLC columns, Preparative recovery and purification of proteins and nucleic acids, GC analysis of hydrocarbons and terpenoid compounds.
Production of enzymes and specialty chemicals in microgravity-based bioreactors, Proficiency in isolation and derivation of natural products, Plant gene expression and molecular biology. Demonstrated experience in maintaining and running medicinal plant cell cultures in bioreactors with emphasis on sampling automation and bioreactor monitoring and control
Broad experience in modern instrumentation and analytical techniques including: HPLC, GPC, SDS-PAGE, GLC, Chromatography, and Computer automation / data analysis. Cell culturing techniques using microgravity-based bioreactors.
Select Research Publications
Valluri, J.V., Bioreactor Culture of Kantikari Cells: Growth and Biomass Characteristics. Biotechnology Letters (in review).
Valluri J.V., B.G. Cobb, R.J. Newton and E.J. Soltes. Changes in Protein and messenger RNA synthesis in slash pine hypocotyls upon pitch canker fungal infection. Journal of Phytopathology, 1999.
Valluri J.V., R.J. Newton and B.G. Cobb. Synthesis of new host coded proteins in Pinus elliottii seedlings in response to pathogen and water stress. Journal of Phytopathology Vol 120 pp 235-244, 1995.
Valluri J.V. Santalum Album L. (Sandalwood): In Vitro Culture and the Bioreactor Production of Secondary Metabolites. Biotechnology, Vol 28, 401- 411,1994.
Valluri J.V., and D.B. Chambers. Protein synthesis in sandalwood callus cultures exposed to drought and salt stress. Plant Physiology and Biochemistry, Vol. 99, (5)
51 - 52, 1992.
Valluri J.V., W.J. Treat and E.J. Soltes. Bioreactor culture of heterotrophic sandalwood (santalum album L.) cell suspensions utilizing a cell-lift impeller. Plant Cell Reports, Vol 10: 366-370, 1991.
Valluri J.V. and E.J. Soltes. Callose formation during wound-inoculated reaction of Pinus elliottii to Fusarium Subglutinans. Phytochemistry, Vol 29 (1), 71-72, 1990.
Valluri J.V., W.J. Treat, J. Castillion and E.J. Soltes. Comparison of photomixotrophic and heterotrophiccallus and suspension cultures of Pinus elliottii: II. Water stress-induced changes in callus volume and protein profiles. Plant Physiology and Biochemistry, Vol 28, (1) 57-64, 1990.
W.M. Alexander and J.V. Valluri. Biofuel potential of Euphorbia Lathyris. Current Science Vol 4, 69-80, 1990.
Valluri J.V., J. Castillion, R.J. Newton and E.J. Soltes. Water stress induced changes in protein synthesis of Pinus elliottii hypocotyls. Journal of Plant Physiology, Vol 135, 355-360, 1989.
Valluri J.V., W.J. Treat, E.J. Soltes, R.J. Newton and B.G. Cobb. Protein synthesis in slash pine callus cultures exposed to water stress. Tree Physiology, Vol 4 (2), 181-186, 1988.
Technical Reports
Valluri, J.V. Environmental Assessment, Belpre Riverfront Park Feasibility Study, Belpre, OH, U.S. Army Corps of Engineers, 2003.
Valluri, J.V., Environmental Assessment and Analysis, City of Parkersburg, OH, U.S. Army Corps of Engineers, 2002.
Valluri, J.V. Environmental Assessment, Island Creek Basin, Logan, WV, U.S. Army Corps of Engineers, 2001.
Valluri, J.V. Environmental Assessment, Grundy Flood Protection / Highway Upgrade Project, Grundy, Virginia, U.S. Army Corps of Engineers, 1999.
Valluri, J.V. Environmental Assessment, Section 14 Planning and Design Analysis for Streambank Protection, Lift Stations, and Sewer Line, South Charleston, WV, U.S. Army Corps of Engineers, 1998.
Valluri, J.V. (Co-author) Environmental Assessment, London Lock Expansion, U.S. Army Corps of Engineers, 1997.
Valluri, J.V. (Co-author) Environmental Assessment, Point Pleasant River Front Park Expansion, U.S. Army Corps of Engineers, 1996.
Valluri, J.V. (Co-author) Draft Environmental Impact Statement (FEIS), Spring Hills Subdivision, FmHA & U.S. Army Corps of Engineers, 1994.
Valluri, J.V. (Co-author) Environmental Impact Statement, Kanawha River Navigation Study, Marmet Lock Replacement, U.S. Army Corps of Engineers, 1993.
Valluri, J.V. (Co-author) Environmental Studies, Appendix C, Marmet Lock Replacement, U.S. Army Corps of Engineers, 1993.
Presentations at National, Regional and State Level Professional Meetings
Valluri J.V., M. Bartholomew and S. R. Gonda. Production of Biomass and Protein for Advanced Life Support in a Microgravity-based Hydrodynamic Focusing Bioreactor (HFB). NASA Cell Science Conference, Galveston, Texas Feb 23, 2005.
Valluri J.V., M. Bartholomew and S. R. Gonda. Selective extraction of bio-active compounds from plant cell cultures in a microgravity-based Hydrodynamic Focusing Bioreactor (HFB). NASA Cell Science Conference, Palo Alto, CA Feb 27, 2004.
Valluri J.V., M. Bartholomew and S.Hoppe. Plant Cell Culture in a Hydrodynamic Focusing Bioreactor (HFB). NASA Cell Science Conference, Houston, Feb 21, 2003.
Valluri J.V. and S. Ibrahim. “Regulation of gene expression in gravistimulated pea stems, Pisum sativum, Tulane University, New Orleans, April 5, 2001.
Holt, T., Valluri J.V., "Characterization of molecular responses in gravistimulated pea stems". WV Academy of Science, April 10, 1999, Shepherd College, Shepherdstown, WV. (Received best paper award from an overall 26 papers)
J.V. Valluri, H.W. Elmore, "Bandanalyzer: software for determination of protein Rf values and calculation of molecular weight". A.S.B. (Association of Southeastern Biologists), April 15, 1999, The University of North Carolina, Wilmington.
Deraimo, D., Britt A, Valluri J.V., H.W. Elmore, "Protein synthesis and stress ethylene production in sandalwood callus cultures exposed to heat shock". WV Academy of Science, April 10, 1999, Shepherd College, Shepherdstown, WV. (Honorable mention - most outstanding paper)
Barnett, J., Valluri J.V., H.W.Elmore, "Protein synthesis and ethylene biosynthesis in sandalwood callus cultures exposed to drought and heat shock". A.S.B. (Association of Southeastern Biologists), April 18, 1997, Furman University, SC. (Regional)
Barnett, J., Valluri J.V., "Protein synthesis in sandalwood callus cultures exposed to drought and heat shock. WV Academy of Science April 12, 1997, WV State College). (State).
Barnett J., J.V. Valluri. Ethylene production in sandalwood callus cultures exposed to drought and heat shock. A.S.B. (Association of Southeastern Biologists), April 11, 1996, Georgia Southern University, Statesboro, Georgia. (Regional)
Barnett J., J.V. Valluri, Campbell J., H.W.Elmore, "Changes in protein synthesis and ethylene production in sandalwood callus cultures exposed to heat shock". A.S.B. (Association of Southeastern Biologists), April 20, 1995, The University of Tennessee, Knoxville. (Regional). (Received best paper award for the Plant Physiology Section)
Barnett,J., J.V. Valluri, J. Marcum and H.W. Elmore, " Protein synthesis in sandalwood callus cultures in response to drought and heat shock". WV Academy of Science, Fairmont State College, Fairmont, WV. April 23, 1994. (State). (Received best paper award from overall 44 papers).
Hannah J., J.V. Valluri and H.W. Elmore, "Protein synthesis in sandalwood callus cultures exposed to drought and salt stress". Second Annual Sigma Xi Research Day. Marshall University, Huntington, WV. March 1992.
Chambers D.B. and J.V. Valluri. "Changes in Protein patterns in drought exposed sandalwood calli". First Annual Sigma XI Research Day. Marshall University, Huntington, WV. March 1991.
Van Buijtenen J.P. and J.V. Valluri. " Genetic differences in needle waxes in loblolly pine and their relation to drought stress ". Tenth North American Forest Biology Workshop, Vancouver, B.C., July 20-22, 1988. (National)
Valluri J.V., T.J. Newton and E.J. Soltes, " Induction of stress proteins in hypocotyls of slash pine (Pinus elliottii) ", Southern section ASPP annual meeting, March 1987. (Regional)
Valluri J.V., C. Hulbert, R.J. Newton. B.G. Cobb, E.J. Soltes, " Characterization of molecular responses in slash and loblolly pines due to pathogen and water stress", Forest Genetics Meetings, USDA Forest Service, Berkeley, California, May 1987(National).
Valluri J.V., E.J. Soltes, R.J. Newton, J. Castillion, " Induction of stress proteins in Pinus elliottii", presented at the symposium of Genetic Manipulation of Woody Plants, E. Lansing, Michigan, June 1987. (National)
Valluri J.V., R.J. Newton, B.G. Cobb, E.J. Soltes, "Induction of new host coded proteins in slash pine subjected to water and pathogen stress", presented at the 19th Southern Tree Improvement Conference, College Station, Texas, June 1987 (Regional).
Valluri J.V. " Changes in gene expression in slash pine in response to disease and water stress" Texas Horticultural Society, Texas A&M University, College Station, Texas, Dec 1987 (Regional).
Valluri J.V. " Role of biotechnology in forest tree improvement", United State Department of Agriculture (USDA), Texas A&M University, College Station, Texas, Dec 1987 (National).
External Grants Awarded
Title: Protein Production for Biological Life Support Systems. Agency: NASA Amount $32,000. Effective dates: 5/1/07-6/30/008.
Title: Production of Biomass and Protein for Advanced Life Support in a Microgravity-based Hydrodynamic Focusing Bioreactor (HFB). Agency: NASA Amount $25,000. Effective dates: 5/1/05-5/30/06.
Title: Characterization of HFB space bioreactor. Agency: USRA’s Division of Space Life Sciences. Amount $10,000. Effective dates: 10/2/04-3/15/05
Title: Production of Biomass and Protein for Advanced Life Support in a Microgravity-based Hydrodynamic Focusing Bioreactor (HFB)
Agency: NASA Amount $28,000. Effective dates: 6/1/05-6/30/06
Title: Plant Cell Culture Growth in a Microgravity-based Bioreactor. Agency: NASA Amount $25,000. Effective dates: 7/1/03-12/15/04
Title: Plant Cell Growth in HFB. Agency: USRA’s Division of Space Life Sciences. Amount $10,000. Effective dates: 9/2/02-3/15/03
Title: Environmental Impact Analysis, Belpre Riverfront Park
Agency: U.S. Army Corps of Engineers; Amount: $25,000
Effective Dates: 3/1/2002– 12/31/2002
Title: Bioreactor Production of Secondary Metabolites from Plant Cell Cultures
Agency: USDA-NRI; Amount: $25,000
Effective Dates: 10/1/2000 – 5/30/2001
Title: Developing Study Components for Environmental Analysis
Agency: U.S. Army Corps of Engineers; Amount: $49,707
Effective Dates: 9/1/2000 – 9/31/2001
Title: Characterization of E. Coli and Salmonella at Beech Fork, East Lynn & Summersville Lakes.
Agency: North American Waterfowl Association, Amount: $3000
Effective Dates: 5/16/2001 -8/31/2001
Title: Geese Management Study at Beech Fork, East Lynn and Summersville Lakes
Agency: NASA; Amount: $2000
Effective Dates: 9/1/2000-12/1/2000
Title: Regulation of Gene Expression in Gravistimulated Pea Stems.
Agency: NASA; Amount: $25,000
Effective Dates: 8/1/1998 - 5/30/2001
Title: Bioproduction of Secondary Metabolites from Plant Cell Cultures.
Agency: Durwood L. Pate Foundation; Amount: $32,000
Effective Dates: 7/1/1998 - 12/30/1999
Title: Alteration of Gene Expression During Drought Stress.
Agency: NASA; Amount: $5000, Effective Dates: Jan 1997 - Dec 1997.
Title: Integration of Molecular Methodology, Recombinant DNA Technology and Computer Applications for Science Majors (with Dr. Wayne Elmore).
Agency: WV Legislature Technology Advantage Grant
Amount: $80,000; Effective Dates: 3/1/1997 - 5/30/1998
Title: Development of Environmental Studies for the Ohio River Main Stem
Agency: U.S. Army Corps of Engineers
Amount: $37,700; Effective Dates: 5/1/97 - 8/31/98
Title: Protein Synthesis and regulation of ethylene in sandalwood callus cultures: Response to heat shock and drought stress.
Agency: NASA; Amount: $2000, Effective Dates: Nov 1995 - May 1996
Title: Enzyme Linked Immuno Assays Using Microplate Reader
Agency: Alfred P. Sloan Foundation
Amount: $10,000; Effective Dates: 1995 - 1996
Title: Development of Environmental Impact Statement for Levisa Fork
Agency: U.S. Army Corps of Engineers
Amount: $20,600; Effective Dates: 6/1/96 - 4/30/97
Title: Environmental Assessment and Analysis for Haysi
Agency: U.S. Army Corps of Engineers
Amount: $20, 800; Effective Dates: 1/1/95 -12/31/95
Title: Development of Systems Impact Modeling and Mitigation Plans for the Marmet Lock Replacement Study.
Agency: U.S. Army Corps of Engineers
Amount: $22,000; Effective Dates: 1/3/94 -12/31/94
Title: Development of Environmental Impact Statement for Marmet Lock Study
Agency: U.S. Army Corps of Engineers
Amount: $ 27,900; Effective Dates: 8/1/92 - 12/31/93
Internal Grants
Marshall University Faculty Research Development Grant, “Gene Expression during Gravistimulation”, $2000, Summer 1998.
Marshall University, College of Science Merit Award, $1000, Dec 1997.
Marshall University Faculty Research Development Grant, "Molecular responses in slash pine induced by drought stress”, $2000, Summer 1997.
Marshall University Faculty Research Development Grant, “Gene expression in pine cells”, $2000, Summer 1996.
Marshall University, Inco Faculty Development Grant, Fall 1995, $2000
Marshall University Greatest Needs Request, Spring 1992, "Changes in protein synthesis in sandalwood callus exposed to NaCl and mannitol,” $2000
Marshall University Faculty Research Grant, "Altered gene expression in slash pine induced by drought stress, $2000, Summer 1990.
Marshall University, Inco Faculty Development Grant, Fall 1995, $2,500
Marshall University Greatest Needs Request, Fall 1990, "Changes in protein synthesis in sandalwood callus exposed to NaCl and mannitol," $2,250.
