CHM 678 APPLIED MICROSCOPY IN RESEARCH SPRING 2019

posted 1-18-2019
8-bit image lecture
example labs-anonymous
lab report format handout
images for FIJI/imageJ lab report -trans light of mouse egg with gold labeled villi -sem image of same type of mouse egg - fluorescent confocal of autofluor sandalwood callus - afm image of CdS nanowire with dna origami on top in 2 formats- SEM image of spider silk - Rotary park, summer 2015 - AFM image of DNA origami with tetrahedron feature

 

posted 2-19-2019
suggested readings in SEM text goldstein 3rd edition
selected reading from Murphy text - one omission as compared to archived version Murphy 2nd edition
SEM beam parameters lab handout

posted 2-26-2019
first SEM lab digital copy
, use diagrams and text to guide your report

posted 3-1-2019

SEM images for beam params lab

drill bit depth of field guide

 

 

BELOW HERE IS ARCHIVAL - ABOVE IS ACTIVE FOR 2019

syllabus chem 678 2018
syllabus chem 481 2018
materials for first SEM lab posted 1-24-2018

first SEM lab digital copy, use diagrams and text to guide your report

images for first SEM lab- carpenter ants (our ants are likely Camponotus pennsylvanicus) and references for carpenter ants

 

posted 1-30-2018 SEM images of HOPG with salt and CNTs - key included in folder

 

posted 2-10-2018 SEM beam params. images for lab report

 

posted 1-20-2017 digital copy of SEM lab, use diagrams straight from here for your lab report - use this to guide your report

posted 1-20-2017 SEM images from first SEM lab - file names identify animals - remember this report is about SEM primarily, not flies (spot size, working distance, contrast, etc.)

posted 1-30-2017 presumed antennal scale SEM slow scan --------- fast scan

posted 2-13-2017 zipped folder with all SEM beam parameter images, depth of field instructions, and digital copy of SEM lab 2 - save to a folder right click and unzip (the EDS information and spectrum of tungsten carbide drill bit are just FYI but you could use the spectrum in lab if you want)

 

posted 2-23-2016 Norton lectures through 2-23-2016 right click and save in a folder, then unzip to same folder, tell me if there are problems

 

posted 3-9-2016 confocal lab handouts to assist with lab reports - confocal lab 1 - confocal lab 2

posted 3-9-2016 LeicaSP5 materials in order of importance SP5 scan head diagram - best confocal overview from Leica - quick start guide for SP5 from Leica - good Olympus LSCM intro

- big LeicaSP5 manual

 

 

 

 

OLD - 2014 and before below

Lectures and images from week 1 posted 2-2016

OLD 2014 AFM lab materials for
afm lecture
mm8 -intro chapter 4

 

OLD, 2014 posted
lectures for weeks 1-7
AFM extras from Paul West of Agilent

OLD, 2014 SEM images for first SEM lab report posted
Reed lab supplied Silicon chips with Au microwires (made by photolithography) and Au nanowires (made by ebeam lithography)

Al sample stub for discussion of contrast and resolution